Evolution is biology’s great Big Idea. It is the lens through which facts are interpreted, as well as the vantage point by which new data are judged. While physicists go on searching for their great unifying Theory of Everything, biologists have essentially had it since
Medicine, of course, falls within the biological sciences, but sometimes you’d almost never guess. This very interesting graphic [“Map of Science”], done in 2007, illustrates the citation flow between disciplines within science. As you can see, there is much crosstalk between the disciplines of medicine and molecular and cell biology, and a considerable exchange of citations between medicine and neuroscience, but the communication link between evolution and medicine is in fact so tenuous that it isn’t even depicted.
Does this matter? Well, evolutionary theory offers the ability to view and answer questions from an ultimate, rather than a proximate vantage point. Both sets of questions are equally valid, but they are different. Think, for instance, of the famous case of the sickle cell allele. The proximate explanation, provided for us by molecular and cell biologists, is of course that this disease results from a single nucleotide mutation in the beta globin gene. As a result of this, a glutamate is replaced by a valine in the transcribed protein, and the haemoglobin molecule risks insoluble polymerisation at low oxygen tensions.
But to stop there would sell us short. The ultimate explanation for sickle cell anaemia operates on quite another level. Significantly, sickle cell disease is an autosomal recessive trait, and to an evolutionist that opens up an entire vista of probabilities. Why is this damaging recessive allele maintained in the population? The main part of the answer is well-known: heterozygous individuals on the whole produce enough normal haemoglobin to avoid symptoms, but they are significantly more resistant to malaria. Thus the recessive gene, far from being eliminated, is maintained in what geneticists call a balanced polymorphism, since the heterozygous phenotype leads to positive selection pressure on the allele, whereas the homozygous phenotype leads to negative selection pressure. This fact neatly explains the impressive correlation between the historical distribution of malaria [Malaria Distribution] and the ancestral distribution of the sickle cell trait [Sickle Cell Distribution].
Randolph Nesse, arguably the leading proponent of evolutionary medicine, illustrated the difference in approaches best, “We are not asking why some people get sick, which is what most medical research asks, but why all humans are vulnerable to disease.” Natural selection is an enormously powerful force, capable of producing an eye that is sensitive to a single photon, a brain capable of love, hate and innumerous calculations and machinations, and body homeostatic processes that make the mind boggle. So why do we then still get sick? And why do we have an appendix, wisdom teeth, a fallible immune system, or an aging body?
Nesse proposed a useful list of 6 reasons for our vulnerability [show a slide!], and it’s this schema that I’ll be concentrating on. My aim is to introduce some of the many evolutionary theories of disease to those who haven’t yet heard them.
The first two categories centre on the idea that natural selection, whilst an enormously powerful force, isn’t necessarily a quick one by our standards. More specifically, the rate of evolutionary change in a population is inversely proportional to the generation time. Our generation time is about 20 to 25 years, which only leaves a handful of generations between here and, the dawn of civilisation – not even close to the number needed to effect significant evolutionary change. The first civilisation is widely believed to be the Sumerian one in
Since the dawn of civilisation our existence has undergone some quite dramatic changes with regards to disease burden, labour types, food types, and countless other trappings. The problem is that 99% of our existence as a species predates this. The result: there are many parts of our bodies that aren’t well-adapted to the modern world, and this explains the first category of disease: “mismatch with the modern environment.”
It is consensus opinion that the rates of allergies and autoimmune diseases have increased markedly in recent times. The rate of increase far has been so rapid that an environmental cause must be at the bottom of it all. Numerous candidates have been proposed, and most centre around the “Hygiene Hypothesis”, which claims that it is paradoxically our rather sanitised modern lifestyles that predispose us to these conditions. Increasingly, however, research is pointing in the direction of the helminths, since cross-sectional studies have shown a consistently negative relationship between helminth infection and allergic diseases. These results have been confirmed by most, though not all, interventional studies. Most of the facts begin to line up when you consider that helminths, if they are common today, were much more so in our evolutionary past. Thus our immune systems evolved with the expectation of a significant helminth load, and the corollary of this is that helminths must have themselves evolved immunomodulatory mechanisms to ensure their own survival. Take away the helminths, however, and the balance is disturbed. Of course, it has long been known that the IgE system is intimately involved with both aspects, but specifics are beginning to come to light too. For instance, in 2007 it was shown that helminths secrete a protein (ES-62) that down-regulates the Type II T cell response. There is also abundant cross-reactivity between antigens on schistosomes and house dust mites.
Interestingly, the immunomodulatory effects of helminths (or rather the lack thereof in today’s 1st world) have also been linked to several autoimmune diseases. For instance, one study in 2005 reported that inflammatory bowel disease patients treated with the sterilized eggs of Trichuris suis improved markedly within months (43% improvement for U.C., 72% improvement for Crohns’). The net has been extended to multiple sclerosis, with a small but well-designed study showing that patients who were recently infected by intestinal helminths had a much, much slower rate of disease progression [MS and Helminths]. The (American) National Multiple Sclerosis Society is presently conducting a phase 2 trial to extend this research. At present, it must be admitted that the evidence is sketchy, but evolutionary insights should never replace hard data anyway. Rather, their role is to offer predictions as to where to look, and what to test for. Theories like the Hygiene Hypothesis can only be helpful, even if they are eventually disproved.
The second reason for our vulnerability disease is concerned with the pathogens that coevolve with us. For instance, since the amount of replication they can do within us is limited, pathogens need an escape route, and they must often temper their virulence in order to meet this requirement. The well-known Spanish Flu epidemic followed hot on the heels of World War I, and the reason is obvious. Influenza is usually a severe but not lethal disease; it needs to keep us alive for long enough in order to spread itself. World War I’s trenches offered another opportunity, though. Suddenly the virus could replicate as fast as it liked without being in significant peril of being stranded within a dying body: as soldiers got sick, they were simply replaced by healthy ones. The constant supply of new replication opportunities (read: soldiers) meant that the check on virulence that had previously been in place now no longer applied.
A similar thing can be confidently predicted with HIV and condom usage. Although condoms obviously significantly decrease HIV transmission (Pope Benedict’s heterodox epidemiological studies notwithstanding), their widespread use can also be expected to lead to the generation of less virulent HIV strains. Again, a virus must be able to spread itself to another host before the host demises, and condom usage means that the average time before it infects someone else will be extended. It follows that its virulence must therefore be somewhat lessened if it is to survive.
Among laypeople, the commonest perceived opinion of why we are vulnerable to diseases is simply that “evolution isn’t strong enough” to rid us of all susceptibilities. As I’ve said, an evolutionary perspective actually lessens the force of this argument – there are so many other possibilities for disease susceptibility other than natural selection’s impotence. But is undeniable that even a force as mighty as shapes eyes and brains has its limits. A nice example comes in the case of deleterious recessive genes. They will be punished appropriately by natural selection, but they must first be seen. As their frequency drops within the gene pool, the selection pressure against them actually drops even faster, with the result being that deleterious recessive genes at low frequencies are very hard to get rid of.
Another example of an evolutionary constraint is the case of the appendix. Most biologists view it as an evolutionary relic (ancestral species would have used a much enlarged version as a pocket to store cellulose-digesting bacteria), but there are some holdouts. Obviously the appendix does perform some functions (it has a large collection of MALT tissue, for instance), but it’s poor logic to claim that any function derived from a tissue is its reason for existence or persistence. The matter is settled by the fact that a patient suffers no demonstrable deficiency of function after an appendicectomy. So why does the troublesome organ persist? Williams and Nesse offered one ingenious hypothesis, which makes note that the appendix only gives us trouble because of its small size. When it was its usual large self in our ancestral past, it was presumably no more likely to become occluded than the next part of the large intestine. Paradoxically, this fact traps evolution, barring it from proceeding any further in the direction of making the appendix smaller, since any decrease in size will actually be punished by increased mortality from appendicitis!
Other interesting evolutionary relics reflect the fact that natural selection can only work with what it is given, and so sometimes comes up with bizarre designs which are only illuminated by looking at our ancestry. The recurrent laryngeal nerve on the left goes on a bizarre walkabout [details from Dawkins/Williams]. … think of how wasteful it is in a giraffe! Other examples include several awkward anatomical patch-jobs that had to be rushed into production as we evolved bipedality. [Show list.]
The fact that our bodies may be largely bundles of compromises is also counter-intuitive. Of course, we’re already a little familiar with the idea: the sickle cell allele is a special kind of trade-off, whereby the best option for evolution in some parts of the world is a partial deficiency! Ageing is perhaps the biggest compromise at all, according to the leading theory of senescence. The first step in understanding why we age is to distinguish between wear-and-tear and senescence. Obviously all things are subject to attrition, but the crucial question is not why this is so, but why this isn’t repaired or overcome. The question isn’t why our molars wear down with age, but why they aren’t replaced. Agelessness isn’t some hopeless dream; it’s amply displayed in cancer cell lines grown around the world, which show no senescence at all. Why aren’t we like that?
Geneticists had long-since noted an interesting fact: the selection pressure on a gene (either positive or negative) necessarily decreases with age, and that is because even without ageing, we wouldn’t be immortal. Sooner or later, a lightening bolt, a fall, a rival or (more likely) a microbe would get us, and so theoretical immortality would never translate into actual immortality. To use an extreme example, therefore, a gene that had an effect at age 999 would not often have the chance to express itself, and so would practically have no selection pressure acting upon it, either positively or negatively.
Williams took this idea to the next level with his brilliant theory of “antagonistic pleitropy”. He first noted that most genes are pleiotropic; that is, they have more than one effect. He then surmised that pleiotropic genes in which positive effects occurred earlier on in life than their negative effects could be selected for - even if their positive traits were outweighed by their negative ones. This is because genes with effects towards the beginning of life would be weighted and counted as more significant, since they were under maximum selection pressure. The result, said Williams (and most researchers since) is ageing.
A vivid example from his original paper is that of a hypothetical gene that increased calcium deposition in the bones, but also gradually in the coronary arteries. Even though enough calcium deposition in the coronary arteries would inevitably be fatal eventually, the selection pressure against this effect would be weak, since it happened late in life when many people would have died from other causes. It is conceivable that such a gene would be selected for since the strong selection pressure towards stronger bones would ‘outvote’ the weaker selection pressure against coronary artery disease.
Such compromises are likely to be legion. For instance (Steven Pinker quotes). Draw distinction between this ultimate explanation and the possible proximate free radical explanation.
The fact that natural selection maximises genetic replication, rather than health, is a counter-intuitive bar to many people’s understanding. As Dawkins has famously noted, the genes in our gonads are the only things that are potentially immortal, and this is the unit of natural selection. Bodies are temporary vehicles that further the replicatory efforts of the genes, rather than the apple of evolution’s eye. ?Again Dawkins: “[Virus vs Elephant quote]”.
Understanding that genetic replication, rather than somatic longevity, is the fundamental aim of evolution, brings into view battlegrounds where we otherwise would never have dreamt to look. Most famous is Trivers and Haig’s idea of the maternal-offspring conflict, which hinges on the fact that mother and child aren’t clones. A mother obviously has 100% of her genes in her, but the fetus only carries half this number. From her genes’ point of view therefore, although the fetus is a valuable bundle of genetic replication, it isn’t the be all and end all. The fetus, with its own set of genes, reasons differently. Specifically, the mother’s genes won’t want her to sacrifice as much of her resources to the fetus as the fetus will want. This [pinker/Williams quote about IGF]. It’s worth noting that this explanation is almost never referred to in medical textbooks.
Lastly we have the “smoke detector principle”, which is that so long as the costs are
cheap, the body will often err on the side of extreme sensitivity in detecting and dealing with potential problems, even when the result is mostly false positives. A smoke detector would rather be wrong 100 times and right about the one true fire than more accurate but miss the fire. So it is with many of our body’s defences. The archetypal example is fever. Although it is usually regarded as merely a troublesome side-effect of infections, the evolutionary evidence says otherwise. Fever is one of the most conserved elements among higher animals, and is the result of an impressively complicated apparatus centred in the anterior hypothalamus. The notion that it is a pathological side-effect of bacteraemia is absolute nonsense when viewed from an evolutionary perspective. The hypothalamic set point has been designed to be sensitive to numerous cytokines involved in the inflammatory process; if this were deleterious on balance, it would be exceptionally easy to prevent. Just stop the hypothalamus from responding to cytokines! Furthermore, we have direct evidence that fever interferes with bacterial enzymes, enhances leukocyte mobility and phagocytosis, and promotes the proliferation of T cells.
A fair few trials have now been done to see whether treatment of fever might actually be harming the patient. The results are contradictory. In some cases, aggressive treatment of fever has been shown to increase mortality, whilst other studies have shown neither this nor a benefit. Why the ambiguous results? Well, the smoke detector principle. The body fires all its guns in the hope that enough of them will hit the target, but fever is often one that misses. Fever seems most effective against bacteria, but the body dutifully raises the hypothalamic set point, just in case, to anything that causes the fairly non-specific cytokines to be released. Therefore, fever can accompany certain conditions where there is likely no benefit, such as viral infections (e.g. influenza), or inflammation from non-infectious causes (e.g. burns). Even within the bacterial group, there are likely certain organisms or situations where fever has a greater effect than in others. However, the smoke detector principle ensures that fever is so non-specific as to make our general studies underpowered. What is necessary is to conduct much more targeted research. Does fever help in acute pneumonias, or any bacteraemia? If so, which organisms does it help most for? Once we are able to answer questions like these, we will have a very clear idea of when to treat fever (which is undeniably uncomfortable) and when to let it run its course. The evolutionary viewpoint can guide us in this research by assuring us that there is a point to fever, even if we don’t yet know what it is.
One little anecdote to end. In my first year studying medicine, I was told by my lecturer that fever is beneficial under certain circumstances. About a week later I got quite sick and was diagnosed with influenza. Doggedly determined to stick it out, I declined all treatment, and was confined to my room for three days with a temperature of around 39°C. I alternated with cycles of shivering and then sweating, and it was one of the worst few days I’ve had over the last decade. Nonetheless, I was consoled that at least I had least shortened the duration of my illness. Now there are all sorts of stupid things about that story, but nothing was quite as disheartening as when I did microbiology in second year, and learnt that influenza was caused by a virus. Fever, thanks to the smoke detector principle, was probably incidental and useless to me.
Thank you.
draft
· Mismatch – hygiene hypothesis for allergies, autoimmune diseases
· Pathogens coevolving with hosts – E. coli vs humans (?citrate example) – evolutionary rationale for sex & HLA-discordant partners
· Constraints on what selection can do (? Appendicitis)
· Trade-offs (malaria, ageing)
· Selection maximises genetic replication, not health (mother-child conflicts in the womb à maternal diabetes, GPH, ?my own theory)
· Smoke-detector principle (my pyretic response to viral influenza)